基于ATR-FTIR结合UPLC-DAD技术鉴别百合及其类似品(1)

http://www.100md.com 2020年1月1日 《中国中医药信息杂志》 20201

     摘要：目的 基于傅里叶变换衰减全反射红外光谱(ATR-FTIR)结合超高效液相色谱-二极管阵列检测器(UPLC-DAD)技术快速区分不同品种百合药材粉末。方法 采用ATR-FTIR采集百合样品红外光谱数据并导入Simca软件，建立正交偏最小二乘判别分析(OPLS-DA)模型，在此基础上，联用UPLC-DAD技术测定正品百合、伪品金百合及微波干燥卷丹中王百合苷A含量，分析其差异性。结果 OPLS-DA二维得分图及层次聚类分析图对卷丹与百合、卷丹与伪品金百合、热风干燥卷丹与微波干燥卷丹進行了良好的分类。卷丹的王百合苷A含量为2.09～3.28 mg/g，百合的王百合苷A含量为1.36～1.77 mg/g，伪品金百合的王百合苷A含量为1.03～1.05 mg/g，微波干燥卷丹的王百合苷A含量为3.92～4.01 mg/g。卷丹和百合中的王百合苷A含量高于伪品金百合，微波干燥卷丹的王百合苷A含量高于普通热风干燥卷丹。结论 本研究建立的方法操作简单、方便、快速，可为百合饮片、百合药膳产品开发及相关药材的质量控制提供借鉴。

    关键词：百合;傅里叶变换衰减全反射红外光谱;超高效液相色谱-二极管阵列检测器;王百合苷A
, 百拇医药
    中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2020)01-0057-06

    DOI：10.3969/j.issn.1005-5304.201901219

Identification of Lilii Bulbus and Its Counterfeit by ATR-FTIR and UPLC-DAD

YUAN Zhiying^1，2，3， LI Dongyang¹， LI Yalin¹， GUAN He¹， LIU Xiangdan¹， XIE Mengzhou^1，3， HUANG Huiyong^1，3

1. Hunan University of Chinese Medicine， Changsha 410208， China; 2. Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces， Changsha 410208， China; 3. Hunan Engineering Technology Center of Functional Food Homology of Medicine， Changsha 410208， China
, 百拇医药
    Abstract： Objective To quickly distinguish different kinds of Lilii Bulbus powder based on ATR-FTIR and UPLC-DAD. Methods The infrared spectral data of samples were collected by ATR-FTIR， and the data was imported into Simca software to establish the orthogonal partial least squares discriminant analysis (OPLS-DA) model. On this basis， UPLC-DAD was used to determine the content of regaloside A in genuine Lilii Bulbus and Lilium trompeten (counterfeit) and microwave dried Lilium lancifolium Thunb.， and the differences among different samples were analyzed. Results Two-dimensional score map of OPLS-DA and hierarchical cluster analysis were used to classify the three groups of Lilium lancifolium Thunb. and Lilium broumii F.E. Brown var.viridulum Baker， Lilium lancifolium Thunb. and Lilium trompeten (counterfeit)， hot-air dried Lilium lancifolium Thunb. and microwave dried Lilium lancifolium Thunb. The content of regaloside A in Lilium lancifolium Thunb. was 2.09–3.28 mg/g， 1.36–1.77 mg/g in Lilium broumii F.E. Brown var.viridulum Baker， 1.03–1.05 mg/g in Lilium trompeten (counterfeit)， and 3.92–4.01 mg/g in microwave Lilium lancifolium Thunb. The content of regaloside A in Lilium lancifolium Thunb. and Lilium broumii, http://www.100md.com(袁志鹰 李东洋 李亚林 管贺 刘湘丹 谢梦洲 黄惠勇)

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